Contact Information
| Email: | jeremy.balsbaugh@uconn.edu |
|---|---|
| Address: | 75 N. Eagleville Road Unit 3149 Storrs, CT 06269-3149 |
Proteomics & Metabolomics Facility (PMF)
Facility Details
The Proteomics & Metabolomics Facility implements ultra-high performance liquid chromatography (UPLC) coupled to high resolution mass spectrometry (MS) to unambiguously identify proteins, peptides and small molecules in complex mixtures.
Email: jeremy.balsbaugh@uconn.edu
Address: 75 N. Eagleville Road Unit 3149 Storrs, CT 06269-3149
Products & Services Available
| Category | Product Name | Description | Reservable |
|---|---|---|---|
| Sample Preparation: Proteomics | Chymotrypsin (PO572111) - 1 vial | 1 vial of Pierce sequencing grade | No |
| Sample Preparation: Metabolomics | Custom sample preparation for metabolomics (hr) | Time spent conducting sample preparation for metabolomics | No |
| Sample Preparation: Proteomics | Custom sample preparation for proteomics (hr) | Time spent conducting custom sample preparation (e.g. BCA protein quantification). Reagents billed separately. | No |
| Sample Preparation: Proteomics | Endoproteinase AspN (PRV1621) - Feb 2025 Batch | PRV1621 ordered from Fisher on PO613537 | No |
| Sample Preparation: Proteomics | Endoproteinase LysC (PI 90051, PO333304) - 1 vial | 1 vial of endoproteinase LysC from Pierce | No |
| Hourly Personnel Fees | Experiment set-up fee for assisted metabolomics analysis | Includes instrument calibration(s), LC solvent changes, column cleaning, QC and blank injections. For assisted runs, this includes sample transfer to injection vials and establishing sample queue(s). | No |
| Hourly Personnel Fees | Experiment set-up fee for assisted proteomics analysis | Includes instrument calibration(s), LC solvent changes, column cleaning, QC and blank injections. For assisted runs, this includes sample transfer to injection vials and establishing sample queue(s). | No |
| Hourly Personnel Fees | Experiment set-up fee for unassisted metabolomics analysis | Includes instrument calibration(s), LC solvent changes, column cleaning, QC and blank injections. | No |
| Sample Preparation: Proteomics | FY25 BCA Assay Reagent Costs | PO586024, Pierce BCA kit for 250 assays, does not include personnel time | No |
| Sample Preparation: Metabolomics | FY25 Waters Total Recovery Vials | 1 pack of Waters P/N 186000384C purchased by PMF on PO 594915. | No |
| Sample Preparation: Proteomics | Filter-Aided Sample Preparation (FASP) | includes reduction, alkylation, tryptic digestion, peptide clean-up | No |
| UPLC-MS/MS analysis: Proteomics | Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) assisted analysis (hr) | PMF-assisted analysis of intact proteins using HDX-MS methods on the Synapt G2Si with HDX UPLC pumps | No |
| Sample Preparation: Proteomics | In-gel digestion (Coomassie blue- or silver-stained) | *includes destaining, reduction, alkylation, tryptic digestion, peptide extraction and clean-up | No |
| Sample Preparation: Proteomics | In-solution tryptic digestion | includes reduction, alkylation, tryptic digestion, peptide clean-up | No |
| Sample Preparation: Proteomics | In-vitro labeling | TMT reagent cost extra | No |
| UPLC-MS/MS analysis: Proteomics | Intact protein MW measurement by infusion | Intact MWs by infusion on any UPLC-MS/MS instrument | No |
| Sample Preparation: Proteomics | Intact protein/peptide sample clean-up | Protein/peptide desalting and/or buffer exchange using Pierce desalting spin columns or MWCO filters | No |
| Hourly Personnel Fees | Manual analysis for metabolomics data (hr) | Time spent manually analyzing data for metabolomics datasets | No |
| Hourly Personnel Fees | Manual analysis for proteomics data (hr) | Time spent analyzing data for proteomics datasets | No |
| Workshop Registration Fees | March 2025 Introduction to Proteomics 3-Day Workshop Registration | March 2025 Introduction to Proteomics 3-Day Workshop Registration | No |
| Sample Preparation: Proteomics | Offline fractionation | Peptide fractionation using Pierce high pH spin columns | No |
| UPLC-MS/MS analysis: Proteomics | Orbitrap Eclipse UPLC-MS/MS (hr) | UPLC-MS/MS analysis on the Orbitrap Eclipse | No |
| Sample Preparation: Proteomics | PTM enrichment | PTM columns billed separately | No |
| Sample Preparation: Proteomics | Pierce HSA/IgG depletion kit (Qty: 24 columns) | Ordered by Evan Patev in Biosupply on Feb 11, 2025 order # | No |
| UPLC-MS/MS analysis: Proteomics | Q Exactive HF UPLC-MS/MS analysis (hr) | UPLC-MS/MS analysis on the Q Exactive HF | No |
| Sample Preparation: Proteomics | S-Trap | includes reduction, alkylation, tryptic digestion, peptide clean-up | No |
| UPLC-MS/MS analysis: Metabolomics | Small molecule measurement by infusion | MS analysis for a non-protein/peptide analyte using infusion on any UPLC-MS/MS instrument | No |
| UPLC-MS/MS analysis: Metabolomics | Synapt G2-Si UPLC-MS/MS metabolomics analysis (hr) | PMF-assisted UPLC-MS/MS runtime on the Synapt G2-Si | Yes |
| Hourly Personnel Fees | User training (hr) | Time spent training users on sample preparation, UPLC-MS/MS, and/or data analysis | No |
| UPLC-MS/MS analysis: Metabolomics | Xevo G2-XS UPLC-MS/MS metabolomics analysis (hr) | PMF-assisted UPLC-MS/MS runtime on the Xevo G2-XS | Yes |
| UPLC-MS/MS analysis: Proteomics | timsTOF HT UPLC-MS/MS analysis (hr) | UPLC-MS/MS analysis using the timsTOF HT | No |
| UPLC-MS/MS analysis: Metabolomics | timsTOF HT UPLC-MS/MS metabolomics analysis (hr) | PMF-assisted UPLC-MS/MS analysis on the timsTOF HT | No |