Contact Information
Email: jeremy.balsbaugh@uconn.edu
Address: 75 N. Eagleville Road Unit 3149 Storrs, CT 06269-3149

The Proteomics & Metabolomics Facility implements ultra-high performance liquid chromatography (UPLC) coupled to high resolution mass spectrometry (MS) to unambiguously identify proteins, peptides and small molecules in complex mixtures.

Proteomics & Metabolomics Facility (PMF)

Products & Services Available

Category Product Name Description Reservable
Sample Preparation: Proteomics Chymotrypsin (PO572111) - 1 vial 1 vial of Pierce sequencing grade No
Sample Preparation: Metabolomics Custom sample preparation for metabolomics (hr) Time spent conducting sample preparation for metabolomics No
Sample Preparation: Proteomics Custom sample preparation for proteomics (hr) Time spent conducting custom sample preparation (e.g. BCA protein quantification). Reagents billed separately. No
Sample Preparation: Proteomics Endoproteinase LysC (PI 90051, PO333304) - 1 vial 1 vial of endoproteinase LysC from Pierce No
Hourly Personnel Fees Experiment set-up fee for assisted metabolomics analysis Includes instrument calibration(s), LC solvent changes, column cleaning, QC and blank injections. For assisted runs, this includes sample transfer to injection vials and establishing sample queue(s). No
Hourly Personnel Fees Experiment set-up fee for assisted proteomics analysis Includes instrument calibration(s), LC solvent changes, column cleaning, QC and blank injections. For assisted runs, this includes sample transfer to injection vials and establishing sample queue(s). No
Hourly Personnel Fees Experiment set-up fee for unassisted metabolomics analysis Includes instrument calibration(s), LC solvent changes, column cleaning, QC and blank injections. No
Sample Preparation: Proteomics Filter-Aided Sample Preparation (FASP) includes reduction, alkylation, tryptic digestion, peptide clean-up No
UPLC-MS/MS analysis: Proteomics Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) assisted analysis (hr) PMF-assisted analysis of intact proteins using HDX-MS methods on the Synapt G2Si with HDX UPLC pumps No
Sample Preparation: Proteomics In-gel digestion (Coomassie blue- or silver-stained) *includes destaining, reduction, alkylation, tryptic digestion, peptide extraction and clean-up No
Sample Preparation: Proteomics In-solution tryptic digestion includes reduction, alkylation, tryptic digestion, peptide clean-up No
Sample Preparation: Proteomics In-vitro labeling TMT reagent cost extra No
UPLC-MS/MS analysis: Proteomics Intact protein MW measurement by infusion Intact MWs by infusion on any UPLC-MS/MS instrument No
Sample Preparation: Proteomics Intact protein/peptide sample clean-up Protein/peptide desalting and/or buffer exchange using Pierce desalting spin columns or MWCO filters No
Hourly Personnel Fees Manual analysis for metabolomics data (hr) Time spent manually analyzing data for metabolomics datasets No
Hourly Personnel Fees Manual analysis for proteomics data (hr) Time spent analyzing data for proteomics datasets No
Workshop Registration Fees March 2025 Introduction to Proteomics 3-Day Workshop Registration March 2025 Introduction to Proteomics 3-Day Workshop Registration No
Sample Preparation: Proteomics Offline fractionation Peptide fractionation using Pierce high pH spin columns No
UPLC-MS/MS analysis: Proteomics Orbitrap Eclipse UPLC-MS/MS (hr) UPLC-MS/MS analysis on the Orbitrap Eclipse No
Sample Preparation: Proteomics PTM enrichment PTM columns billed separately No
UPLC-MS/MS analysis: Proteomics Q Exactive HF UPLC-MS/MS analysis (hr) UPLC-MS/MS analysis on the Q Exactive HF No
Sample Preparation: Proteomics S-Trap includes reduction, alkylation, tryptic digestion, peptide clean-up No
UPLC-MS/MS analysis: Metabolomics Small molecule measurement by infusion MS analysis for a non-protein/peptide analyte using infusion on any UPLC-MS/MS instrument No
UPLC-MS/MS analysis: Metabolomics Synapt G2-Si UPLC-MS/MS metabolomics analysis (hr) PMF-assisted UPLC-MS/MS runtime on the Synapt G2-Si Yes
Hourly Personnel Fees User training (hr) Time spent training users on sample preparation, UPLC-MS/MS, and/or data analysis No
UPLC-MS/MS analysis: Metabolomics Xevo G2-XS UPLC-MS/MS metabolomics analysis (hr) PMF-assisted UPLC-MS/MS runtime on the Xevo G2-XS No
UPLC-MS/MS analysis: Proteomics timsTOF HT UPLC-MS/MS analysis (hr) UPLC-MS/MS analysis using the timsTOF HT No
UPLC-MS/MS analysis: Metabolomics timsTOF HT UPLC-MS/MS metabolomics analysis (hr) PMF-assisted UPLC-MS/MS analysis on the timsTOF HT No