Contact Information
Email: bo.reese@uconn.edu
Address:

Center for Genome Innovation (CGI)

Facility Details

The mission of the CGI is to provide state-of-the-art expertise in genome technologies while facilitating genomics research for faculty and students across the University of Connecticut campuses.

Email: bo.reese@uconn.edu

Address:

Products & Services Available

Category Product Name Description Reservable
Library Preparation Services - Illumina Compatible Amplicon High Throughput Library Prep This alternative sample processing scheme is meant for batch processing of samples in sets of 48. Samples will only be processed in sets of 48 and will be combined with an additional 2, 48-sample sets of samples for a high throughput MiSeq sequencing run. High throughput MiSeq sequencing will be 500 cycle v2 with a total of 48x3 sample sets. No
Labor and Data Processing Amplicon PCR low throughput labor rate for Singlet, Doublet or Triplet PCR 1 + 2 ONLY Low throughput amplicon PCR labor charge for library prep. No sample minimum. Ideal for samples less than 48 samples. No
Library Preparation Services - Illumina Compatible Amplicon sequencing DOUBLET PCR1 + PCR2 Amplicon sequencing for a variety of amplicon targets using fusion primers for 2-step PCR barcoding. Doublet PCR1 reaction per sample plus PCR2 No
Library Preparation Services - Illumina Compatible Amplicon sequencing PCR2 only Amplicon sequencing for a variety of amplicon targets using fusion primers for 2-step PCR barcoding. PCR2 barcoding only for pre-amplified PCR products. No
Library Preparation Services - Illumina Compatible Amplicon sequencing SINGLET PCR1 + PCR2 Amplicon sequencing for a variety of amplicon targets using fusion primers for 2-step PCR barcoding. Single PCR1 reaction per sample plus PCR2 No
Library Preparation Services - Illumina Compatible Amplicon sequencing TRIPLET PCR1 + PCR2 Amplicon sequencing for a variety of amplicon targets using fusion primers for 2-step PCR barcoding. Triplicate PCR1 reaction per sample plus PCR2 No
Labor and Data Processing Data Processing Fee Hourly fee for sequencing data processing, management and QC. No
Equipment Usage Equipment Usage - Agilent Femto Pulse Per sample equipment charge for use of the Agilent Femto Pulse system. No
Equipment Usage Equipment Usage - Agilent Tapestation 4200 Per sample equipment charge for use of the Agilent 4200 Tapestation system. No
Equipment Usage Equipment Usage - Illumina MiSeq Per sample equipment cost for instrument usage, added on for each sample. No
Labor and Data Processing External Processing Fee 35% external processing fee based on internal rate total for project No
Sample QC Femto Pulse 165Kb DNA Fragment Analysis Long Read sample QC and library preparation instrument for precise length assessment of genomic DNA samples. Ideal for sample QC with intent to run on the PacBio Revio or Oxford Nanopore systems. No
Library Preparation Services - Illumina Compatible ILMN Stranded mRNA Ligation - Library prep RNA-seq library preparation method for intact total RNA, DNA-free, w/ polyA selection included. Cost includes Agilent tapestation total RNA QC and final library QC. No
Labor and Data Processing Labor per sample HT 1-Step, DNA submitted HT amplicon, 1-step, Either DNA for MARS 16s/ITS or amplicon for 2-step No
Labor and Data Processing Labor per sample HT 2-Step, DNA submitted Amplicon High Throughput Labor Charge per sample, 2-step No
Sample QC Library Pool Screening Check MiSeq Reagent kit Nano v2 300 cycle kit for checking pooling evenness and library clustering prior to deep sequencing. NOTE: Cost of flow cell may be shared if pooling on a single lane with other samples No
Labor and Data Processing Low throughput Amplicon PCR Labor rate for PCR 2 ONLY Amplicon processing for PCR2 only. Ideal for low throughput sample processing of samples less than 48 samples. No
Sequencing MiSeq MiSeq Reagent Kit v2 500 cycle Cost includes Illumina shipping and temporary tariff fee No
Sequencing MiSeq MiSeq Reagent Kit v2 Nano, 300 cycle Illumina sequencing kit for 1M clusters. 300 cycles of sequencing. Cost includes Illumina shipping (6.5%) and the temporary tariff charge of (5%). Total of 11.5% added on to it cost. No
Sequencing NovaSeq X NovaSeq Xplus 10B lane sequencing, 300 cycle Single lane sequencing for paired end 150bp reads. 10B flow cell yield is 2.5B total PE reads per lane. No
Sequencing NovaSeq X NovaSeq Xplus Library QC NovaSeq Xplus sequencing library QC charges per pool No
Sample Extraction Qiagen MagAttract on Kingfisher plate of 48 High throughput semiautomated extraction services for 48 samples or more. No
Labor and Data Processing Qiagen MagAttract on Kingfisher plate of 48 - Labor charge per sample Per sample labor charge added on per sample when processing full plate. No
Labor and Data Processing RNAseq library prep per sample labor Per sample labor charge for all RNA-seq lirbary prep methods including Illumina Stranded and Takara mRNA and Picov2. No
Sample Extraction Sample Extraction for RNA Total RNA isolation from cell pellets, lysed tissues or saliva. Column based extraction method used No
Sample QC Tapestation D1000 - Regular Sensitivity For sample QC of high concentration DNA samples less than 1000bp No
Sample QC Tapestation D5000 - Regular Sensitivity DNA sample QC assay for molecules up to 5000bp. Regular sensitivity intended for higher concentration samples. No
Sample QC Tapestation Genomic DNA For QC of genomic DNA samples. Not ideal for long read sequencing applications No
Sample QC Tapestation High Sensitivity D1000 Low concentration assay intended for samples less than 25ng/uL with fragments less than 1000bp No
Sample QC Tapestation High Sensitivity D5000 DNA sample QC assay for low concentration samples with molecules up to 5000bp. No
Sample QC Tapestation High Sensitivity RNA For QC of total RNA samples 25ng/uL or less No
Labor and Data Processing Total RNA Extraction Labor - Flat rate Labor charge for extraction services - flat rate No