Contact Information
Email: bo.reese@uconn.edu
Address:

Center for Genome Innovation (CGI)

Facility Details

The mission of the CGI is to provide state-of-the-art expertise in genome technologies while facilitating genomics research for faculty and students across the University of Connecticut campuses.

Email: bo.reese@uconn.edu

Address:

Products & Services Available

Category Product Name Description Reservable
Library Preparation Services - Illumina Compatible 10X Genomics Chromium Library Prep Consumables Per sample consumables charge for completion of customer prepared cDNA samples from the 10X Chromium platform. Customer to supply all library preparation reagents from 10X Genomics. No
Reagent Replacement 10X Genomics Chromium™ i7 Multiplex Kit, 96 rxns Index well barcode reactions used for library preparation. Costs are the same for plates SI-TT or SI-NT. No
Library Preparation Services - Illumina Compatible Amplicon High Throughput Library Prep This alternative sample processing scheme is meant for batch processing of samples in sets of 48. Samples will only be processed in sets of 48 and will be combined with an additional 2, 48-sample sets of samples for a high throughput MiSeq sequencing run. High throughput MiSeq sequencing will be 500 cycle v2 with a total of 48x3 sample sets. No
Labor and Data Processing Amplicon Initial Bioinformatics Fastq to OTU using mothur. No
Labor and Data Processing Amplicon PCR low throughput labor rate for Singlet, Doublet or Triplet PCR 1 + 2 ONLY Low throughput amplicon PCR labor charge for library prep. No sample minimum. Ideal for samples less than 48 samples. No
Library Preparation Services - Illumina Compatible Amplicon sequencing DOUBLET PCR1 + PCR2 Amplicon sequencing for a variety of amplicon targets using fusion primers for 2-step PCR barcoding. Doublet PCR1 reaction per sample plus PCR2 No
Library Preparation Services - Illumina Compatible Amplicon sequencing PCR2 only Amplicon sequencing for a variety of amplicon targets using fusion primers for 2-step PCR barcoding. PCR2 barcoding only for pre-amplified PCR products. No
Library Preparation Services - Illumina Compatible Amplicon sequencing SINGLET PCR1 + PCR2 Amplicon sequencing for a variety of amplicon targets using fusion primers for 2-step PCR barcoding. Single PCR1 reaction per sample plus PCR2 No
Library Preparation Services - Illumina Compatible Amplicon sequencing TRIPLET PCR1 + PCR2 Amplicon sequencing for a variety of amplicon targets using fusion primers for 2-step PCR barcoding. Triplicate PCR1 reaction per sample plus PCR2 No
Labor and Data Processing CGI Hourly Labor Hourly labor add-on for CGI services. No
Labor and Data Processing DNA for Enrichment - Human Exome Labor Charge Flat rate for library preparation processing. No
Library Preparation Services - Illumina Compatible DNA for Enrichment - Human Exome library prep The Illumina DNA Prep with Exome 2.5 Enrichment kit provides library prep and enrichment reagents using the Twist Bioscience for Illumina Exome 2.5 Panel. No
Labor and Data Processing Data Analysis and Consultation Hourly custom data analysis. No
Labor and Data Processing Data Processing Fee Hourly fee for sequencing data processing, management and QC. No
Equipment Usage Equipment Usage - Agilent Femto Pulse Per sample equipment charge for use of the Agilent Femto Pulse system. No
Equipment Usage Equipment Usage - Agilent Tapestation 4200 Per sample equipment charge for use of the Agilent 4200 Tapestation system. No
Equipment Usage Equipment Usage - Illumina MiSeq Per sample equipment cost for instrument usage, added on for each sample. No
Equipment Usage Equipment Usage - NovaSeq 6000 Illumina NovaSeq 6000 Equipment Usage fee charged per sample. No
Equipment Usage Equipment Usage - PacBio Revio Equipment cost per SMRT cell for usage on the Revio No
Labor and Data Processing External Processing Fee 35% external processing fee based on internal rate total for project No
Sample QC Femto Pulse 165Kb DNA Fragment Analysis Long Read sample QC and library preparation instrument for precise length assessment of genomic DNA samples. Ideal for sample QC with intent to run on the PacBio Revio or Oxford Nanopore systems. No
Library Preparation Services - Illumina Compatible ILMN Stranded mRNA Ligation - Library prep RNA-seq library preparation method for intact total RNA, DNA-free, w/ polyA selection included. Cost includes Agilent tapestation total RNA QC and final library QC. No
Labor and Data Processing ISOSeq Kinnex Labor Library Prep Labor charge for sample preparation. Flat rate for samples up to 48 samples No
Library Preparation Services - Illumina Compatible Illumina custom Primer Plate Kozich based primers for 16S v4 (EMP or HMP), ITS2, or 2step No
Labor and Data Processing Labor - NEBNext UltraII FS DNA Library Preparation Per sample lirbary prep charge for whole genome shotgun library preparation. No
Labor and Data Processing Labor - PAXGene Blood Total RNA Extraction Labor rate for sample processing for PAXGene Blood tubes. Flat Rate per 96-well plate. No
Labor and Data Processing Labor per sample HT 1-Step, DNA submitted HT amplicon, 1-step, Either DNA for MARS 16s/ITS or amplicon for 2-step No
Labor and Data Processing Labor per sample HT 2-Step, DNA submitted Amplicon High Throughput Labor Charge per sample, 2-step No
Sample QC Library Pool Screening Check MiSeq Reagent kit Nano v2 300 cycle kit for checking pooling evenness and library clustering prior to deep sequencing. NOTE: Cost of flow cell may be shared if pooling on a single lane with other samples No
Library Preparation Services - Illumina Compatible Low Input Pico Total-RNA library preparation SMART-Seq Total RNA Pico Input (ZapR Mammalian) is designed for efficient preparation of Illumina sequencing libraries from picogram input amounts (250 pg–10 ng) of total RNA. Technologies included in this product enable processing of high-quality, partially degraded, or low-quality RNA, including RNA prepared from FFPE or LCM samples No
Labor and Data Processing Low throughput Amplicon PCR Labor rate for PCR 2 ONLY Amplicon processing for PCR2 only. Ideal for low throughput sample processing of samples less than 48 samples. No
Sequencing MiSeq MiSeq Reagent Kit Micro, v2 300 cycle Illumina MiSeq Micro flow cell for approximately 8M total PE reads per run. Paired end sequencing up to 150bp each read. Cost includes Illumina shipping and temporary 5% tariff surcharge. No
Sequencing MiSeq MiSeq Reagent Kit v2 500 cycle Cost includes Illumina shipping and temporary tariff fee No
Sequencing MiSeq MiSeq Reagent Kit v2 Nano, 300 cycle Illumina sequencing kit for 1M clusters. 300 cycles of sequencing. Cost includes Illumina shipping (6.5%) and the temporary tariff charge of (5%). Total of 11.5% added on to it cost. No
Long Read Sequencing Minion Flowcell Minion Flowcell No
Library Preparation Services - Illumina Compatible NEBNext UltraII FS DNA Library Preparation Library preparation costs per sample for whole genome shotgun library prep. Kit can be used for metagenome library preparation, standard WGS and aDNA sample prep. Cost includes all third party reagents and consumables needed, including sample barcoding, sample QC and final library QC. No
Sequencing NovaSeq X NovaSeq Xplus 10B lane sequencing, 200 cycle NovaSeq Xplus 10B flow cell contains 8 sequencing lanes. 200 cycles of sequencing possible. Lanes can be run fully or shared if barcodes do not overlap. Lane cost includes shipping and temporary 5% Illumina Tariff charge. No
Sequencing NovaSeq X NovaSeq Xplus 10B lane sequencing, 300 cycle Single lane sequencing for paired end 150bp reads. 10B flow cell yield is 2.5B total PE reads per lane. No
Sequencing NovaSeq X NovaSeq Xplus 25B lane sequencing, 300 cycle NovaSeq Xplus 25B lane sequencing for 6.5B total PE reads per lane. 8 lanes of sequencing possible on 1 flow cell. Partial lane usage possible. Cost includes library pool QC, Illumina shipping and temporary 5% tariff surcharge. No
Sequencing NovaSeq X NovaSeq Xplus Library QC NovaSeq Xplus sequencing library QC charges per pool No
Illumina Sequencing - NovaSeq 6000 NovaSeq6000 S2 100 cycle S2 flow cell sequencing for 7.4 - 8.0B total PE reads per flow cell. 2 lanes possible. Lane sharing possible using add on of 2-lane Xp kit. Cost includes Illumina shipping and temporary 5% tariff surcharge. No
Illumina Sequencing - NovaSeq 6000 NovaSeq6000 SP 100 cycle SP flow cell sequencing for 1.8 - 2.0B total PE reads per flow cell. 2 lanes possible. Lane sharing possible using add on of 2-lane Xp kit. Cost includes Illumina shipping and temporary 5% tariff surcharge. No
Long Read Library Preparation ONT - 1D Direct ligation (SQK-LSK114) Direct Ligation DNA Library Prep using the Ligation Sequencing Kit V14 (SQK-LSK114) and all necessary 3rd party components. No
Labor and Data Processing ONT - Labor Charge for 1D Direct ligation (SQK-LSK114) Flat rate for sample processing of DNA Direct ligation samples. No
Sample Extraction PAXGene Blood Total RNA Extraction Total RNA isolation including DNase treatment for blood collected in PAXgene tubes. No
Long Read Library Preparation PacBio Kinnex ISO-Seq - 4 PLEX Kinnex ISO-Seq library preparation for PacBio sequencing. 4 PLEX reaction for the Kinnex reagent only. No
Long Read Library Preparation PacBio Kinnex ISO-Seq - 5 PLEX Kinnex ISO-Seq library preparation for PacBio sequencing. 5 PLEX reaction for the Kinnex reagent only. No
Long Read Library Preparation PacBio Kinnex ISO-Seq - 6 PLEX Kinnex ISO-Seq library preparation for PacBio sequencing. 6 PLEX reaction for the Kinnex reagent only. No
Long Read Sequencing PromethION DNA flow cell Oxford Nanopore PromethION DNA flow cells No
Sample QC QiAxcel DNA Screening Row of 1-12 samples screening No
Sample Extraction Qiagen MagAttract on Kingfisher plate of 48 High throughput semiautomated extraction services for 48 samples or more. No
Labor and Data Processing Qiagen MagAttract on Kingfisher plate of 48 - Labor charge per sample Per sample labor charge added on per sample when processing full plate. No
Labor and Data Processing RNAseq library prep per sample labor Per sample labor charge for all RNA-seq lirbary prep methods including Illumina Stranded and Takara mRNA and Picov2. No
Long Read Sequencing Revio SMRT cell sequencing PacBio Revio SMRT cell sequencing. Cost for 1 flow cell and reagents needed for ABC library setup using the Revio polymerase kit No
Sample Extraction Sample Extraction for RNA Total RNA isolation from cell pellets, lysed tissues or saliva. Column based extraction method used No
Library Preparation Services - Illumina Compatible Takara Low Input mRNA library prep SMART-Seq mRNA LP generates oligo(dT)-primed, full-length mRNA-seq libraries. The chemistry is optimized for use on ultra-low amounts of total RNA (10 pg–100 ng, RIN ≥ 8) or for direct use on multiple intact cells (< 1,000 cells) No
Sample QC Tapestation D1000 - Regular Sensitivity For sample QC of high concentration DNA samples less than 1000bp No
Sample QC Tapestation D5000 - Regular Sensitivity DNA sample QC assay for molecules up to 5000bp. Regular sensitivity intended for higher concentration samples. No
Sample QC Tapestation Genomic DNA For QC of genomic DNA samples. Not ideal for long read sequencing applications No
Sample QC Tapestation High Sensitivity D1000 Low concentration assay intended for samples less than 25ng/uL with fragments less than 1000bp No
Sample QC Tapestation High Sensitivity D5000 DNA sample QC assay for low concentration samples with molecules up to 5000bp. No
Sample QC Tapestation High Sensitivity RNA For QC of total RNA samples 25ng/uL or less No
Labor and Data Processing Total RNA Extraction Labor - Flat rate Labor charge for extraction services - flat rate No