The UConn Health Flow Cytometry Facility provides flow cytometric analysis and cell sorting services to all UConn researchers as well as researchers at neighboring institutions. The facility, located on the 6th floor of the E building in room E6014, consists of a 900 square-foot lab space, complete with fume hood, centrifuge, and sink space. The UConn Health Center Flow Cytometry Facility has six instruments available for cellular analysis and two cell sorters. We have 2 BD FACS Symphony A5 spectral edition cell analyzers and these complement our Becton Dickinson (BD) LSR II, our automated high-throughput Bio Rad ZE5, Accuri C6 analyzer, and Amnis ImageStream Mark II. The FACS Symphony A5 SE cell analyzers have 5 lasers, 50 detectors and enable the capability of spectral fluorescence cell analysis while maintaining the ability to perform conventional flow cytometry experiments. Each of these instruments is equipped with a high throughput sampler. Additionally, the facility operates a Bio Rad ZE5, which can obtain samples from 96-well and 384-well culture plates and detect up to 27 different parameters. The Amnis ImageStream Mark II imaging flow cytometer has 4 excitation lasers, 12 imaging channels and 3 objective lenses. It combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone. The facility also houses two BD high speed cell sorters, a FACS ARIA II and a FACS Symphony S6 SE. The BD FACS ARIA II and FACS Symphony S6 SE cell sorters optically match our BD analyzers and can perform 4-way or 6-way sorting, respectively. Both have the option for single cell deposition into multi-well plates. The sorters are also equipped with aerosol containment for sorting biohazardous materials and are entirely enclosed in biosafety cabinets. We also provide several computer workstations with flow cytometry and cytometric bead array analysis software. These include: FlowJo (Treestar Inc.), BD FACS DIVA (BD Bioscience), Cell Quest Pro (BD Bioscience), ModFit LT (Verity), and FCAP Array (BD Bioscience). The facility is staffed Monday through Friday from 9 a.m. to 6 p.m. After hours or weekend access is permitted for trained UConn Health employees. Please contact us with any questions regarding the facility, flow cytometry, or the development of new applications.

Email: Jellison@uchc.edu

Address: 263 Farmington Ave MC1319 Farmington CT 06030